SARS-CoV-2 Spike Glycoprotein S2 extracellular domain (ECD), His Tag

Catalog #: EG-328

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Cat #	EG-328
Description	A recombinant form of spike glycoprotein S2 extracellular domain (ECD, S686 to P1213) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography.
Mutations	WT
Tags	C-terminal hexa-histidine
Purification	FPLC by nickel affinity and ion exchange chromatography
Sequence	1 SVASQSIIAY TMSLGAENSV AYSNNSIAIP TNFTISVTTE ILPVSMTKTS 51 VDCTMYICGD STECSNLLLQ YGSFCTQLNR ALTGIAVEQD KNTQEVFAQV 101 KQIYKTPPIK DFGGFNFSQI LPDPSKPSKR SFIEDLLFNK VTLADAGFIK 151 QYGDCLGDIA ARDLICAQKF NGLTVLPPLL TDEMIAQYTS ALLAGTITSG 201 WTFGAGAALQ IPFAMQMAYR FNGIGVTQNV LYENQKLIAN QFNSAIGKIQ 251 DSLSSTASAL GKLQDVVNQN AQALNTLVKQ LSSNFGAISS VLNDILSRLD 301 KVEAEVQIDR LITGRLQSLQ TYVTQQLIRA AEIRASANLA ATKMSECVLG 351 QSKRVDFCGK GYHLMSFPQS APHGVVFLHV TYVPAQEKNF TTAPAICHDG 401 KAHFPREGVF VSNGTHWFVT QRNFYEPQII TTDNTFVSGN CDVVIGIVNN 451 TVYDPLQPEL DSFKEELDKY FKNHTSPDVD LGDISGINAS VVNIQKEIDR 501 LNEVAKNLNE SLIDLQELGK YEQYIKWPAH HHHHHH
Mol Wt	59.0 kDa
Source	HEK 293
Purity	90~95% by SDS-PAGE
Applications	Screening of neutralizing antibodies, western blot, ELISA, animal vaccination
Storage Buffer	PBS + 50% Glycerol
Storage	-20ºC ~ -80ºC
Shipping	dry ice
References

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