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SARS-CoV-2 Spike Glycoprotein S2 extracellular domain (ECD), His Tag

Catalog #: EG-328


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Cat # EG-328
Description A recombinant form of spike glycoprotein S2 extracellular domain (ECD, S686 to P1213) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography.
Mutations
WT
Tags
C-terminal hexa-histidine
Purification
FPLC by nickel affinity and ion exchange chromatography
Sequence
1   SVASQSIIAY TMSLGAENSV AYSNNSIAIP TNFTISVTTE ILPVSMTKTS
51  VDCTMYICGD STECSNLLLQ YGSFCTQLNR ALTGIAVEQD KNTQEVFAQV
101 KQIYKTPPIK DFGGFNFSQI LPDPSKPSKR SFIEDLLFNK VTLADAGFIK
151 QYGDCLGDIA ARDLICAQKF NGLTVLPPLL TDEMIAQYTS ALLAGTITSG
201 WTFGAGAALQ IPFAMQMAYR FNGIGVTQNV LYENQKLIAN QFNSAIGKIQ
251 DSLSSTASAL GKLQDVVNQN AQALNTLVKQ LSSNFGAISS VLNDILSRLD
301 KVEAEVQIDR LITGRLQSLQ TYVTQQLIRA AEIRASANLA ATKMSECVLG
351 QSKRVDFCGK GYHLMSFPQS APHGVVFLHV TYVPAQEKNF TTAPAICHDG
401 KAHFPREGVF VSNGTHWFVT QRNFYEPQII TTDNTFVSGN CDVVIGIVNN
451 TVYDPLQPEL DSFKEELDKY FKNHTSPDVD LGDISGINAS VVNIQKEIDR
501 LNEVAKNLNE SLIDLQELGK YEQYIKWPAH HHHHHH
Mol Wt
59.0 kDa
Source
HEK 293
Purity
90~95% by SDS-PAGE

Applications

Screening of neutralizing antibodies, western blot, ELISA, animal vaccination
Storage Buffer

PBS + 50% Glycerol

Storage -20ºC ~ -80ºC
Shipping dry ice
References


     
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