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SARS-CoV-2 Spike Glycoprotein, HexaPro Stabilized Trimer, His and Twin-Strep Tags

Catalog #: EG-325


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Cat # EG-325
Description A recombinant form of the full length spike glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography. This recombinant protein has been stabilized by several mutations: removing the polybasic S1/S2 cleavage site by changing RRAR to GSAS (residues 682 to 685), KV to PP mutations (residues 986 and 987), and 4 additional proline substitutions, producing a more stable HexaPro variant (K986P, V987P, F817P, A892P, A899P and A942P). T4 foldon trimerization domain and Octa-histidine tag is located at the C-terminus. This protein forms a trimer, which is suitable for screening of neutralizing antibodies in vitro
Mutations
R682G, R683S, A684A, R685S, K986P, V987P, F817P, A892P, A899P and A942P
Tags
C-terminal Octa-histidine and Twin-Strep tags
Purification
FPLC by nickel affinity and ion exchange chromatography
Sequence
1    CVNLTTRTQL PPAYTNSFTR GVYYPDKVFR SSVLHSTQDL FLPFFSNVTW
51   FHAIHVSGTN GTKRFDNPVL PFNDGVYFAS TEKSNIIRGW IFGTTLDSKT
101  QSLLIVNNAT NVVIKVCEFQ FCNDPFLGVY YHKNNKSWME SEFRVYSSAN
151  NCTFEYVSQP FLMDLEGKQG NFKNLREFVF KNIDGYFKIY SKHTPINLVR
201  DLPQGFSALE PLVDLPIGIN ITRFQTLLAL HRSYLTPGDS SSGWTAGAAA
251  YYVGYLQPRT FLLKYNENGT ITDAVDCALD PLSETKCTLK SFTVEKGIYQ
301  TSNFRVQPTE SIVRFPNITN LCPFGEVFNA TRFASVYAWN RKRISNCVAD
351  YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG DEVRQIAPGQ
401  TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
451  ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS
501  FELLHAPATV CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLPFQQ
551  FGRDIADTTD AVRDPQTLEI LDITPCSFGG VSVITPGTNT SNQVAVLYQD
601  VNCTEVPVAI HADQLTPTWR VYSTGSNVFQ TRAGCLIGAE HVNNSYECDI
651  PIGAGICASY QTQTNSPGSA SSVASQSIIA YTMSLGAENS VAYSNNSIAI
701  PTNFTISVTT EILPVSMTKT SVDCTMYICG DSTECSNLLL QYGSFCTQLN
751  RALTGIAVEQ DKNTQEVFAQ VKQIYKTPPI KDFGGFNFSQ ILPDPSKPSK
801  RSPIEDLLFN KVTLADAGFI KQYGDCLGDI AARDLICAQK FNGLTVLPPL
851  LTDEMIAQYT SALLAGTITS GWTFGAGPAL QIPFPMQMAY RFNGIGVTQN
901  VLYENQKLIA NQFNSAIGKI QDSLSSTPSA LGKLQDVVNQ NAQALNTLVK
951  QLSSNFGAIS SVLNDILSRL DPPEAEVQID RLITGRLQSL QTYVTQQLIR
1001 AAEIRASANL AATKMSECVL GQSKRVDFCG KGYHLMSFPQ SAPHGVVFLH
1051 VTYVPAQEKN FTTAPAICHD GKAHFPREGV FVSNGTHWFV TQRNFYEPQI
1101 ITTDNTFVSG NCDVVIGIVN NTVYDPLQPE LDSFKEELDK YFKNHTSPDV
1151 DLGDISGINA SVVNIQKEID RLNEVAKNLN ESLIDLQELG KYEQGSGYIP
1201 EAPRDGQAYV RKDGEWVLLS TFLGRSLEVL FQGPGHHHHH HHHSAWSHPQ
1251 FEKGGGSGGG GSGGSAWSHP QFEK 
Mol Wt
140.7kDa
Source
HEK 293
Purity
90~95% by SDS-PAGE

Applications

Screening of neutralizing antibodies, western blot, ELISA,  animal vaccination
Storage Buffer

PBS + 50% Glycerol

Storage -20ºC ~ -80ºC
Shipping dry ice
References


     
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