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SARS-CoV-2 Spike Glycoprotein, Stabilized Trimer, His tag

Catalog #: EG-303


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Cat # EG-303
Description A recombinant form of the full length spike glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography. This recombinant protein has been stabilized by several mutations:  removing the polybasic S1/S2 cleavage site (RRAR to A, residues 682 to 685); K986P and V987P;  adding a T4 foldon trimerization domain followed by a hexa-histidine tag at the C-terminus. This protein forms a trimer, which is suitable for screening of neutralizing antibodies in vitro
Mutations
(682PRAR685)->A, K986P, V987P
Tags
C-terminal hexa-histidine
Purification
FPLC by nickel affinity and ion exchange chromatography
Sequence
1    CVNLTTRTQL PPAYTNSFTR GVYYPDKVFR SSVLHSTQDL FLPFFSNVTW
51   FHAIHVSGTN GTKRFDNPVL PFNDGVYFAS TEKSNIIRGW IFGTTLDSKT
101  QSLLIVNNAT NVVIKVCEFQ FCNDPFLGVY YHKNNKSWME SEFRVYSSAN
151  NCTFEYVSQP FLMDLEGKQG NFKNLREFVF KNIDGYFKIY SKHTPINLVR
201  DLPQGFSALE PLVDLPIGIN ITRFQTLLAL HRSYLTPGDS SSGWTAGAAA
251  YYVGYLQPRT FLLKYNENGT ITDAVDCALD PLSETKCTLK SFTVEKGIYQ
301  TSNFRVQPTE SIVRFPNITN LCPFGEVFNA TRFASVYAWN RKRISNCVAD
351  YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG DEVRQIAPGQ
401  TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
451  ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS
501  FELLHAPATV CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLPFQQ
551  FGRDIADTTD AVRDPQTLEI LDITPCSFGG VSVITPGTNT SNQVAVLYQG
601  VNCTEVPVAI HADQLTPTWR VYSTGSNVFQ TRAGCLIGAE HVNNSYECDI
651  PIGAGICASY QTQTNSPASV ASQSIIAYTM SLGAENSVAY SNNSIAIPTN
701  FTISVTTEIL PVSMTKTSVD CTMYICGDST ECSNLLLQYG SFCTQLNRAL
751  TGIAVEQDKN TQEVFAQVKQ IYKTPPIKDF GGFNFSQILP DPSKPSKRSF
801  IEDLLFNKVT LADAGFIKQY GDCLGDIAAR DLICAQKFNG LTVLPPLLTD
851  EMIAQYTSAL LAGTITSGWT FGAGAALQIP FAMQMAYRFN GIGVTQNVLY
901  ENQKLIANQF NSAIGKIQDS LSSTASALGK LQDVVNQNAQ ALNTLVKQLS
951  SNFGAISSVL NDILSRLDPP EAEVQIDRLI TGRLQSLQTY VTQQLIRAAE
1001 IRASANLAAT KMSECVLGQS KRVDFCGKGY HLMSFPQSAP HGVVFLHVTY
1051 VPAQEKNFTT APAICHDGKA HFPREGVFVS NGTHWFVTQR NFYEPQIITT
1101 DNTFVSGNCD VVIGIVNNTV YDPLQPELDS FKEELDKYFK NHTSPDVDLG
1151 DISGINASVV NIQKEIDRLN EVAKNLNESL IDLQELGKYE QYIKWPSGRL
1201 VPRGSPGSGY IPEAPRDGQA YVRKDGEWVL LSTFLGHHHH HH
Mol Wt
137.6 kDa
Source
HEK 293
Purity
90~95% by SDS-PAGE

Applications

Screening of neutralizing antibodies, western blot, ELISA,  animal vaccination
Storage Buffer

PBS + 50% Glycerol

Storage -20ºC ~ -80ºC
Shipping dry ice
References


     
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