SARS-CoV-2 Spike Glycoprotein S1 Domain, His Tag

Catalog #: EG-326

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Cat #	EG-326
Description	A recombinant form of spike glycoprotein S1 domain (V16 to R685) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography.
Mutations	WT
Tags	C-terminal hexa-histidine
Purification	FPLC by nickel affinity and ion exchange chromatography
Sequence	1 VNLTTRTQLP PAYTNSFTRG VYYPDKVFRS SVLHSTQDLF LPFFSNVTWF 51 HAIHVSGTNG TKRFDNPVLP FNDGVYFAST EKSNIIRGWI FGTTLDSKTQ 101 SLLIVNNATN VVIKVCEFQF CNDPFLGVYY HKNNKSWMES EFRVYSSANN 151 CTFEYVSQPF LMDLEGKQGN FKNLREFVFK NIDGYFKIYS KHTPINLVRD 201 LPQGFSALEP LVDLPIGINI TRFQTLLALH RSYLTPGDSS SGWTAGAAAY 251 YVGYLQPRTF LLKYNENGTI TDAVDCALDP LSETKCTLKS FTVEKGIYQT 301 SNFRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY 351 SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT 401 GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE 451 RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF 501 ELLHAPATVC GPKKSTNLVK NKCVNFNFNG LTGTGVLTES NKKFLPFQQF 551 GRDIADTTDA VRDPQTLEIL DITPCSFGGV SVITPGTNTS NQVAVLYQDV 601 NCTEVPVAIH ADQLTPTWRV YSTGSNVFQT RAGCLIGAEH VNNSYECDIP 651 IGAGICASYQ TQTNSPRRAR SGSHHHHHH
Mol Wt	76.1 kDa
Source	HEK 293
Purity	90~95% by SDS-PAGE
Applications	Screening of neutralizing antibodies, western blot, ELISA, animal vaccination
Storage Buffer	PBS + 50% Glycerol
Storage	-20ºC ~ -80ºC
Shipping	dry ice
References

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