T7 DNA Polymerase

Catalog #: EG-1059

Qty:

Description	T7 DNA Polymerase catalyzes DNA synthesis in the 5'->3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a very strong 3'->5' exonuclease activity towards single- and double-stranded DNA.
Applications	Purification of covalently closed circular DNA by removal of residual genomic DNA Primer extension reactions on long templates DNA 3'-end labeling Strand extensions in site-directed mutagenesis Fill-in blunting of 5'-overhang DNA Second strand synthesis of cDNA In situ detection of DNA fragmentation associated with apoptosis
Source	T7 DNA Polymerase is composed of two subunits: an 80 kDa polypeptide (the product of gene 5 of bacteriophage T7) and a 12 kDa thioredoxin from the trxA gene of E. coli. Each protein is cloned and overexpressed separately in E. coli.
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.
Components	T7 DNA Polymerase: 10,000 U/ml in 50 mM KPO₄, 0.1 mM EDTA, 1.0 mM DTT, 50% glycerol, pH 7.0 @ 25°C 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM MgCl₂ 10 mM DTT, pH 7.5 @ 25°C
Quality Control	The absence of endodeoxyribonucleases is confirmed by the appropriate quality test.
Storage Condition	-20 °C

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