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T7 DNA Polymerase

Catalog #: EG-1059


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Description

T7 DNA Polymerase catalyzes DNA synthesis in the 5'->3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a very strong 3'->5' exonuclease activity towards single- and double-stranded DNA.

Applications
  • Purification of covalently closed circular DNA by removal of residual genomic DNA
  • Primer extension reactions on long templates
  • DNA 3'-end labeling
  • Strand extensions in site-directed mutagenesis
  • Fill-in blunting of 5'-overhang DNA
  • Second strand synthesis of cDNA
  • In situ detection of DNA fragmentation associated with apoptosis
Source T7 DNA Polymerase is composed of two subunits: an 80 kDa polypeptide (the product of gene 5 of bacteriophage T7) and a 12 kDa thioredoxin from the trxA gene of E. coli. Each protein is cloned and overexpressed separately in E. coli.
Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.
Components
  • T7 DNA Polymerase: 10,000 U/ml in 50 mM KPO4, 0.1 mM EDTA, 1.0 mM DTT, 50% glycerol, pH 7.0 @ 25°C
  • 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM MgCl2 10 mM DTT, pH 7.5 @ 25°C
Quality Control The absence of endodeoxyribonucleases is confirmed by the appropriate quality test.
Storage Condition -20 °C

     
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