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T4 DNA Polymerase

Catalog #: EG-1058


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Description

T4 DNA Polymerase catalyzes the extension of a primed DNA template (in the presence of template and primer) in the 5′→ 3′ direction. This enzyme exhibits a powerful 3′→ 5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli), while lacking any inherent 5′→ 3′ exonuclease or strand displacement functions.

Applications
  • Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs
  • Blunting of PCR products with 3'-dA overhangs
  • Single strand deletion subcloning (Ligation-independent cloning of PCR products).
  • Second strand synthesis in site-directed mutagenesis.
  • Probe labeling using replacement synthesis.
Source A recombinant E. coli strain carrying a cloned gene 43 of bacteriophage T4.
Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.
Components
  • T4 DNA Polymerase: 3,000 U/ml in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 6.5 @ 25°C
  • 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT, pH 7.9 @ 25°C
Quality Control The absence of endodeoxyribonucleases confirmed by appropriate quality tests.
Storage Condition

-20 °C


     
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