Log In | Shopping Cart | Advanced Search
            
  • Home
  • Career
  • Distributors
  • Contact Us:
  • Email: crm@excellgen.com
Products
Modified mRNA
Encapsulated mRNA
mRNA Enzyme
SARS-CoV-2
Pseudovirus
Popular Products
Cancer Proteins
Antibodies
DNA Binding Proteins
Electrophoresis
Genome Engineering
Polymerases
Recombinases
Transcription Factors
Transfection
Comsumbles
Services
Modified mRNA
Custom Cloning
Virus Production
Protein Purification
Protein Expression
Polymerases
 Previous 
 Return to List 
 Next 

Schizosaccharomyces pombe Poly(U) Polymerase

Catalog #: EG-151


Please Choose:



Qty:

Description

S. pombe Poly(U) (Poly(A)) Polymerase uses ATP as a substrate for template-independent addition of UMP from UTP or AMP from ATP to the 3´ hydroxyl termini of RNA molecules.

Applications
  • Labeling of RNA with UTP
  • Poly(U) and Poly(A) tailing of RNA for cloning
  • Enhance mRNA stability and increase translation efficiency of in vitro synthesized mRNA transferred into eukaryotic cells
  • Poly (A) tailing of 2' O-Me modified 3' ends
Source A recombinant E. coli strain carrying yeast (Schizosaccharomyces pombe) Poly(A) Polymerase gene Cid1
Unit Definition 1 unit is defined as the amount of enzyme that will incorporate 1 nmol of UMP into into RNA in 50 µl in 10 minutes at 37°C.
Components
  • S. pombe Poly(U) Polymerase: 20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.5 @ 25°C
  • 10X Reaction Buffer: 100 mM Tris-HCl,500 mM NaCl, 100 mM MgCl2,10 mM DTT, pH 7.9 @ 25°C
Quality Control The absence of endonuclease, nicking activity, exonuclease and RNAse activity is confirmed by appropriate quality tests.
Storage Condition

-20 °C

 


Customers who bought this product also purchased...

Yeast Poly(A) Polymerase
Yeast Poly(A) Polymerase
     
 Ask a Question 
 
 Home | Privacy Policy | Terms & Conditions | Online Price Policy | Shipping & Return

Copyright © 2007-2023  Excellgen, Inc. All rights reserved