Log In
|
Shopping Cart
|
Advanced Search
Home
Career
Distributors
Contact Us:
Email: crm@excellgen.com
Products
Modified mRNA
Encapsulated mRNA
mRNA Enzyme
SARS-CoV-2
Pseudovirus
Popular Products
Cancer Proteins
Antibodies
DNA Binding Proteins
Electrophoresis
Genome Engineering
Polymerases
Recombinases
Transcription Factors
Transfection
Comsumbles
Services
Modified mRNA
Custom Cloning
Virus Production
Protein Purification
Protein Expression
Polymerases
Previous
Return to List
Next
T7 RNA Polymerase
Catalog #:
EG-1060
Please Choose:
Size
5,000 Units ( $50.00 )
50,000 Units ( $248.00 )
Qty:
Description
T7 RNA Polymerase catalyzes the 5'->3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from a T7 phage promoter (
TAATACGACTCACTATA
G
GGAGA
).
Applications
Radiolabeled RNA probe preparation
RNA generation for
in vitro
translation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
Source
Purified from a strain of
E. coli
that expresses the recombinant T7 RNA Polymerase gene (gene I)
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Components
T7 RNA Polymerase:
50,000 U/ml in 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C
10X Reaction Buffer
: 400 mM Tris-HCl, 60 mM MgCl
2
, 100 mM DTT, 20 mM, Spermidine, pH 7.9 @ 25°C
Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate tests.
Functionally tested in in vitro transcription reaction.
Storage Condition
-20 °C
Ask a Question
Home
|
Privacy Policy
|
Terms & Conditions
|
Online Price Policy
|
Shipping & Return
Copyright © 2007-2023 Excellgen, Inc. All rights reserved