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Cy5-modified NLS-Cre Recombinase mRNA

Catalog #: EG-355


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Product Name NLS-Cre Recombinase (NLS-Cre) mRNA,  modified with Cyanine 5-UTP (Cy5) and Pseudo-UTP at 1:3 ratio
Description

This NLS-Cre mRNA will express human/mouse codon optimized Cre recombinase fused to nuclear localization sequence. The mRNA can be used for loxP recombination in cell lines and primary cells. The expression of NLS-Cre protein can be visualized by a Cy5 filter. 

Codon Optimization Human & mouse
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at a ratio of 1:3
  • 5mCTP
  • PolyA: 180 nt
Application ex vivo LoxP recombination
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC
  • Agarose gel electrophoresis
  • Activity assay: HEK293 cells transfected with LoxP reporter plasmid
Shipping Dry ice
Storage -80 oC

     
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