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Cy5-modified NLS-Flp Recombinase mRNA

Catalog #: EG-356


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Product Name NLS-FLP Recombinase mRNA,  modified with Cyanine 5-UTP (Cy5) and Pseudo-UTP at 1:3 ratio
Description

This NLS-FLP mRNA will express human codon optimized Flp recombinase fused to nuclear localization sequence. The mRNA can be used for FRT recombination in cell lines and primary cells. The expression of NLS-FLP protein can be visualized by a Cy5 filter. 

Codon Optimization Human
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at a ratio of 1:3
  • 5mCTP
  • PolyA: 180 nt
Application ex vivo FRT recombination
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC
  • Agarose gel electrophoresis
  • Activity assay: HEK293 cells transfected with FRT reporter plasmid
Shipping Dry ice
Storage -80 oC

     
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