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Cy5-modified Firefly Luciferase FLuc mRNA

Catalog #: EG-316


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Product Name Firefly Luciferase mRNA, modified with Cy5
Description This Cy5 modified firefly luciferase mRNA can be visualized under fluorescence microscope using a Cy5 filter for the analysis of delivery efficiency. The expression efficiency can be quantified by luciferase assay
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at a ratio of 1:3
  • PolyA: 180 nt
Application Report assay
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC

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