Log In | Shopping Cart | Advanced Search
            
  • Home
  • Career
  • Distributors
  • Contact Us:
  • Phone: 855-278-2398
  • Email: crm@excellgen.com
Products
SARS-CoV-2
mRNA
Pseudovirus
Popular Products
New Products
Cancer Proteins
Antibodies
Cytokines
DNA Binding Proteins
DNA Modifying Enzymes
Electrophoresis
Genome Engineering
Glucosyltransferases
Glycosylases
Kinases and Phosphatases
Ligases
Lysozymes
Nucleases
Nuclear Receptors
PCR & qPCR
Peptides
Polymerases
Proteases
Recombinant Proteins
Recombinases
Restriction Enzymes
RNA Enzymes
Stem Cell Reagents
Transcription Factors
Transfection
Topoisomerases
Comsumbles
GE AKTA FPLC
Services
Conjugation
Custom Cloning
Virus Production
Protein Expression
DNA Modifying Enzymes
 Previous 
 Return to List 
 Next 

E. coli RecA

Catalog #: EG-1014


Please Choose:



Qty:

Description

RecA is essential for homologous recombination, reactions involving DNA repair and UV-induced mutagenesis in E coli (1,2,3). RecA binds to single stranded DNA, resulting in the polymerization of RecA into a nucleoprotein complex. This complex can align with complementary double stranded DNA, resulting in RecA catalysis of DNA strand exchange. RecA DNA binding is stimulated by ATP hydrolysis or non-hydrolyzable ATP analogs. The RecA-ATP-single stranded DNA complex also can function as a coprotease in the proteolytic cleavage of LexA, UmuD and certain bacteriophage proteins. In a healthy cell, LexA represses the expression of genes encoding DNA repair proteins (SOS genes). Upon injury of DNA, LexA catalyzes its own digestion, thereby allowing synthesis of necessary SOS proteins. However, LexA can only induce self-catalysis when activated by a ssDNA-RecA filament. A single filament will bind and activate several LexA proteins, each of which then cleaves other bound proteins. Thus, ssDNA-RecA, a product of DNA injury, stimulates DNA repair.

Applications
  • Visualization of DNA structures with electron microscopy (3)
  • D-loop mutagenesis (4)
  • Screening libraries using RecA-coated probes (5,6)
  • Cleavage of DNA at any single predetermined site (7,8,9)
  • RecA mediated affinity capture for full length cDNA cloning (10, 11)
Source An E. coli strain that carries the E. coli recA gene
Components

RecA: 2 mg/ml in 10 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 @ 25°C

Quality Control

The absence of exo- and endodeoxyribonucleases, non-specific DNase and RNase is confirmed by appropriate quality test.

Storage Condition

-20 °C

References

  1. West, S.C. (1992). Ann. Rev. Biochem.. 61, 603-640.
  2. Radding, C.M. (1991). J. Biol. Chem.. 266, 5355-5358.
  3. Wasserman, S.A. and Cozzarelli, N.R. (1985). Proc. Natl. Acad. Sci. USA. 82, 1079-1083.
  4. Shortle, D. et al. (1980). Proc. Natl. Acad. Sci. USA. 77, 5375-5379.
  5. Honigberg, S.M. et al. (1986). Proc. Natl. Acad. Sci. USA. 83, 9586-9590.
  6. Rigas, B. et al. (1986). Proc. Natl. Acad. Sci. USA. 83, 9591-9595.
  7. Ferrin, L.J. and Camerini-Otero, R.D. (1991). Science. 254, 1494-1497.
  8. Koob, M. et al. (1992). Nucl. Acids Res.. 20, 5831-5836.
  9. Koob, M. (1992). R. Wu(Ed.), Methods in Enzymology. 216, 321-329. San Diego: Academic Press.
  10. Zhumabayeva, B. et al. (1990). Biotechniques. 27, 834-845.
  11. Zhumabayeva, B. et al. (2001). Biotechniques. 30, 512-520.

Customers who bought this product also purchased...

Turbo TEV protease
Turbo TEV protease
Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts
Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts
Thermus thermophilus Muts DNA mismatch repair protein
Thermus thermophilus Muts DNA mismatch repair protein
Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag
Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag
     
 Ask a Question 
 
 Home | Privacy Policy | Terms & Conditions | Online Price Policy | Shipping & Return

Copyright © 2007-2021  Excellgen, Inc. All rights reserved