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DNA Modifying Enzymes

Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag

Catalog #: EG-25

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Product Name	Thermus aquaticus MutS DNA mismatch repair protein
Description	The Taq MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This Muts protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Taq MutS is active at temperature between 0 to 75°C. Since Taq MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels
Applications	Remove mismatch DNA (error correction) from gene synthesis reaction Mutation detection and removal Rapid isothermal SNP detection
Source	E. coli
Fusion Tag	None
Purification Method	FPLC
Purity	~ 95% as determined by SDS-PAGE
Accession #	AAC43637, TAU3311, U33117
Gene Name	Thermus aquaticus MutS DNA mismatch repair protein
Protein Sequence	1 MEGMLKGEGP GPLPPLLQQY VELRDQYPDY LLLFQVGDFY ECFGEDAERL ARALGLVLTH 61 KTSKDFTTPM AGIPLRAFEA YAERLLKMGF RLAVADQVEP AEEAEGLVRR EVTQLLTPGT 121 LLQESLLPRE ANYLAAIATG DGWGLAFLDV STGEFKGTVL KSKSALYDEL FRHRPAEVLL 181 APELLENGAF LDEFRKRFPV MLSEAPFEPE GEGPLALRRA RGALLAYAQR TQGGALSLQP 241 FRFYDPGAFM RLPEATLRAL EVFEPLRGQD TLFSVLDETR TAPGRRLLQS WLRHPLLDRG 301 PLEARLDRVE GFVREGALRE GVRRLLYRLA DLERLATRLE LGRASPKDLG ALRRSLQILP 361 ELRALLGEEV GLPDLSPLKE ELEAALVEDP PLKVSEGGLI REGYDPDLDA LRAAHREGVA 421 YFLELEERER ERTGIPTLKV GYNAVFGYYL EVTRPYYERV PKEYRPVQTL KDRQRYTLPE 481 MKEKEREVYR LEALIRRREE EVFLEVRERA KRQAEALREA ARILAELDVY AALAEVAVRY 541 GYVRPRFGDR LQIRAGRHPV VERRTEFVPN DLEMAHELVL ITGPNMAGKS TFLRQTALIA 601 LLAQVGSFVP AEEAHLPLFD GIYTRIGASD DLAGGKSTFM VEMEEVALIL KEATENSLVL 661 LDEVGRGTSS LDGVAIATAV AEALHERRAY TLFATHYFEL TALGLPRLKN LHVAAREEAG 721 GLVFYHQVLP GPASKSYGVE VAAMAGLPKE VVARARALLQ AMAARREGAL DAVLERLLAL 781 DPDRLTPLEA LRLLQELKAL ALGAPLDTMK G
Storage Buffer	20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 1 mM DTT, 50% Glycerol
Reaction Buffer	100mM KCl, 50 mM Tris-HCl, pH 8.5, 5~20 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C
Storage	-20 to -80 °C.
Shipping	4°C or dry ice
References	Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 2004 Nov 23;32(20):e162. Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 2005 Mar 30;33(6):e55. MutS as a tool for mutation detection. Acta Biochim Pol. 2005;52(3):575-83. Epub 2005 Aug 4. One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36. Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods - 4, 257 - 262 (2007) doi:10.1038/nmeth1007

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