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D7324 Tag, Mouse Monoclonal Antibody 1E2

Catalog #: EG-283

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Product Name	D7324 Tag, Mouse Monoclonal Antibody 1E2
Overview	The D7324 tag (APTKAKRRVVQREKR) is derived from the conserved C terminal of HIV-1 gp120 corresponding to amino acids numbers 497-511 of the BH10 strain of HIV-1 (Ratner , L. et al., Nature, 313, 277-284 (1985). The target was first used for capturing HIV-1 envelope with a goat polyclonal antibody D7324 in capture ELISA format. It has then been used as an epitope tag in expression of recombinant soluble membrane proteins (including HIV-1, SIV, Dengue, Zika, and Lassa virus (LASV), et al) for conventional capture ELISA in detection of antibody immune response and monoclonal antibody screening and characterization. Many recombinant viral envelope proteins have been engineered to express C-terminal D7324 tag, which does not appear to interfere with the bioactivity of the recombinant protein. The monoclonal antibody 1E2 can capture C-terminal, D7324-tag fusion proteins as well as many native Env from lysate of HIV-1 virions or infected cells
Clone Number	1E2
Host	Mouse
Class	Recombinant Monoclonal
Immunogen	D7324 tag (APTKAKRRVVQREKR)
Isotype	IgG2a/kappa
Species Cross-Reactivity	N/A
Purity	The antibody was purified from serum-free 293F culture supernatant by Protein A affinity-chromatography
Concentration	~1 mg/ml
Formulation	1 x DPBS, pH 7.4, without additive
QC	50 µl of 4 µg/ml 1E2 mAb in PBS was coated in ELISA plate overnight at 4 ^oC. D7324-tagged protein supernatant from transient transfection of 293F cells was then captured and detected by a protein specific detection antibody.
Shipping	Blue ice
Storage	-20 ^oC or 4 ^oC. Stable for 1 year at -20 ^oC

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