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Anti-B7-H3 monoclonal antibody, recombinant

Catalog #: EG-372

$250.00

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EG-372 Anti-B7-H3-C10

Product Name	Anti-B7-H3 monoclonal antibody, recombinant
Cat #	EG-372
Size	100 µg
Key features	Rabbit monoclonal antibody produced in 293 cells with serum-free, protein-free medium, purified with protein A column; High batch-to-batch consistency and reproducibility; Long term security of supply;
Clone number	C10
Immunogen	Recombinant extracellular domain of B7-H3 that was expressed and purified from 293 cells
Reaction target	Extracellular region of B7-H3
Species Cross-Reactivity	Human
Applications	Flow Cytometry; IP; ELISA;
Working concentrations	Flow cytometry and ELISA: 5 µg/ml of final concentration is suggested. IP: 10 μg in 0.3-0.5 mg cell lysates is suggested.
Background	B7-H3 (also known as CD276) is a member of B7 family, which is over-expressed in many cancer types. B7-H3 is an immune checkpoint protein that inhibits tumor antigen-specific immune responses. B7-H3 can also promote tumor migration, invasion, angiogenesis, chemoresistance, epithelial-to-mesenchymal transition (EMT), and regulate tumor cell metabolism (Kontos F, et al. B7-H3: an attractive target for antibody-based immunotherapy. Clinical Cancer Research. 2020, 27: 1227–1235). B7-H3 is regarded as an attractive target for cancer immunotherapy. Several anticancer agents are targeting B7-H3, such as enoblituzumab (MGA271), omburtamab, MGD009, MGC018, DS-7300a, and CAR T cells.
QC	Flow cytometry assay showing cell surface expression of B7-H3. KLM-1 cells (10^6) were incubated with 5 μg antibody in 1 ml PBS buffer containing 5% BSA. Antibody binding was detected with Cy5-conjugated goat-anti-rabbit antibody. Blue line, isotype control antibody staining; red line, B7-H3 antibody staining.
Concentration	As indicated on the vials
Formulation	80% PBS, pH 7.4; 20% Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative
Shipping	Blue ice
Storage	-20 to -70 ^oC (long term) or 4 ^oC (one week). Depending on the expected frequency of use, the product can be aliquoted to avoid heavily repeated freezing/thawing cycles.

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