Catalog #: EG-376
Flow cytometry analysis using M181 monoclonal antibody. A431 is SIRPa negative cells. A431 (SIRPa++) is derived from A431 that was forcefully over-expressing SIRPa. One million cells were incubated with 5 μg antibody in 1 ml PBS buffer containing 5% BSA. Antibody binding was detected with Cy5-conjugated goat-anti-rabbit antibody. Shade area, isotype control antibody staining; Red line, M181 staining.