Protein Expression using Stable Cell Line
Excellgen uses a novel homologous recombination technology to generate stable cell lines from engineered CHO and 293 cell lines. Using this new technology we can recover targeted cell population as little as 2 weeks, and isolate clones in 3 to 4 weeks. Because the gene of interest is integrated into a predefined transcriptionally active genomic site, predictable transgene expression is guaranteed. Highly stable protein expression has been observed for at least 20 weeks even in the absence of selection agents.
Deliverables
- Stable cell line
- Minimum 3 mg purified recombinant protein, > 95% purity
- QC Data
Required Materials
- 5 µg of plasmid containing gene of interest plus complete vector sequence in electronic format
- or protein sequence for gene synthesis (with additional price for gene synthesis)
- or accession number
Quotation and Ordering
To request a quotation, please request a quotation form from customer services crm@excellgen.com
To order, please send the completed quotation form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone.
If submitting samples, please mail them together with a hard copy of the completed quotation form to: Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743.
