Log In | Shopping Cart | Advanced Search
            
  • Home
  • Career
  • Distributors
  • Contact Us:
  • Email: crm@excellgen.com
Products
Modified mRNA
Encapsulated mRNA
mRNA Enzyme
SARS-CoV-2
Pseudovirus
Popular Products
Cancer Proteins
Antibodies
DNA Binding Proteins
Electrophoresis
Genome Engineering
Polymerases
Recombinases
Transcription Factors
Transfection
Services
Modified mRNA
Custom Cloning
Virus Production
Protein Purification
Protein Expression
Protein Expression
 Previous 
 Return to List 
 Next 

Protein Expression and Purification using Yeast Expression System

Catalog #: EG-1077


Qty:

Excellgen's scientists have average of 15 years experience in molecular biology, protein expression and purification. Our team is proficient in molecular cloning, producing and purifying of recombinant proteins in a variety of expression systems including cell-free protein synthesis, E. Coli, yeasts, baculovirus/insect cells and mammalian cells (CHO and 293). We have ÄKTAExplore and ÄKTAPurifier FPLC systems, ~ 30 large GE Pharmacia prepacked columns (gel filtration, hydrophobic, ion exchange, desalting) and numerous affinity columns. By combining state-of-the-art fementation and ÄKTA FPLC chromatography systems, we can produce quantities of pure recombinant proteins ranging from miligrams to a few grams.

This package is designed for producing and purification of 3 mg recombinant protein in Yeast Expression System.

Timeline Milestones Tasks
2 weeks Cloning and Subcloning
  • PCR amplification using high fiderity DNA polymerase
  • Clone PCR product into an expression vector
  • Restriction digestion analysis and sequencing of the construct to ensure correct sequence and frame
  • Preparation of plasmid DNA
1-2 weeks Pilot Expression and Purification
  • Transform yeast cells with plasmid DNA
  • Small scale expression and purification study assessed by SDS-PAGE, Dot-blot or Western blot analysis
  • Estimation of protein yield
1-2 weeks Large Scale Expression and Purification
  • Large-scale growth of over-expressing yeast cells
  • Purify protein using AKTA FPLC systems
  • Dialysis and concentrate protein
  • SDS-PAGE or Western blot analysis of the purified protein
  • Determination of protein concentration
  • Delivery of purified and ready-to-use protein

Deliverables

  • Minimum 3 mg purified recombinant protein, > 95% purity
  • QC Data
  • Chromatograms

Required Materials

  • 5 µg of plasmid containing gene of interest plus complete vector sequence in electronic format
  • or protein sequence for gene synthesis (with additional price for gene synthesis)
  • or accession number so we can buy cDNA clone from suppliers such as ATCC, Open Biosystems, Origene, GeneCopoeia

Quotation and Ordering


To request a quotation, please request a quotation form from customer services crm@excellgen.com

To order, please send the completed quotation form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone.

If submitting samples, please mail them together with a hard copy of the completed quotation form to: Protein Services, Excellgen, 847 Quince Orchard Blvd, Suite H, Gaithersburg, MD, 20878, US.


     
 Ask a Question 
 
 Home | Privacy Policy | Terms & Conditions | Online Price Policy | Shipping & Return

Copyright © 2007-2022  Excellgen, Inc. All rights reserved