Cy5-modified NLS-Cre Recombinase mRNA

Catalog #: EG-355

Size	Catalog #	Price	Add to Cart
ARCA 10 µg	—	$595.00
ARCA 20 µg	—	$980.00
Cleancap 10 µg	—	$595.00
Cleancap 20 µg	—	$980.00
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Product Description

Product Name	NLS-Cre Recombinase (NLS-Cre) mRNA, Cy5- and Pseudo-UTP-Modified
Overview	This in vitro transcribed NLS-Cre mRNA encodes a human/mouse codon-optimized Cre recombinase fused to a nuclear localization sequence (NLS). The mRNA is designed for efficient ex vivo loxP-mediated recombination in cell lines and primary cells. Incorporation of Cy5-labeled nucleotides enables direct visualization of mRNA delivery and expression via fluorescence microscopy using a Cy5 filter.
Codon Optimization	Human and mouse
Key Features	5' Cap Structure: ARCA (Anti-Reverse Cap Analog; m⁷(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m⁷G(5')ppp(5')(2'-O-methyl-A)pG, Cap 1) for enhanced mRNA stability and translation. Nucleotide Modifications: Cy5-UTP and N¹-methyl-pseudouridine-5'-triphosphate at a 1:3 molar ratio, plus 5-methylcytidine triphosphate (5mCTP), to improve mRNA stability and reduce immunogenicity. Poly(A) Tail: 180 nucleotides for optimal translation efficiency.
Applications	Ex vivo loxP site-specific recombination Visualization of mRNA delivery and expression by Cy5 fluorescence
Concentration	~1 µg/µL
Recommended Transfection Protocol	Lipofectamine^™ MessengerMAX^™ Transfection Reagent Protocol
Quality Control	Agarose gel electrophoresis Functional assay: HEK293 cells transfected with loxP reporter plasmid
Shipping Conditions	Shipped on dry ice
Storage	Store at –80 °C

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