NLS-eCas9 Self-Amplifying mRNA

Catalog #: EG-322
SizeCatalog #PriceAdd to Cart
ARCA 20 µg$980.00
ARCA 100 µg$1980.00
ARCA 1 mg$15000.00
Cleancap 20 µg$980.00
CleanCap 100 µg$1980.00
CleanCap 1 mg$15000.00
CleanCap 5 X 1 mg$72000.00
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Product Description
Product Name NLS-eCas9 Self-Amplifying mRNA
Description

This self-amplifying mRNA encodes four nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and NLS-eCas9.

NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at both N and C terminus. The mutant Cas9 reduces off-target effects by more than 10 folds.

Modifications
  • 5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application CRISPR, Genome Enginering
Concentration ~1 µg/µl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC
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