NLS-Cas9-D10A Nickase mRNA
Catalog #: EG-315
| Size | Catalog # | Price | Add to Cart |
|---|---|---|---|
| ARCA 20 µg | — | $280.00 | |
| ARCA 100 µg | — | $680.00 | |
| ARCA 1 mg | — | $3680.00 | |
| Cleancap 20 µg | — | $280.00 | |
| CleanCap 100 µg | — | $680.00 | |
| CleanCap 1 mg | — | $3680.00 | |
| CleanCap 5 X 1 mg | — | $13180.00 | |
| View Cart | |||
Product Description
| Product Name | NLS-Cas9-D10A Nickase mRNA |
| Description |
NLS-Cas9-D10A Nickase mRNA encodes a Streptococcus pyogenes SF370 Cas9 protein D10A mutant with nuclear localization signal (NLS) at C terminus. Unlike the wild-type CAS9, which cleaves the double strand DNA generating two break sites based on its two active domains, the NLS-Cas9-D10A Nickase has only one active domain, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand break. This mutant mRNA favors homology-directed repair and decreases the occurrence of non-homologous end joining. |
| Modifications |
|
| Application | CRISPR, Genome Enginering |
| Concentration | ~1 µg/µl |
| Transfection Protocol | Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol |
| QC | Agarose gel electrophoresis |
| Shipping | Dry ice |
| Storage | -80 oC |
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