NLS-eCas9-T2A-EGFP mRNA

Catalog #: EG-314
SizeCatalog #PriceAdd to Cart
ARCA 20 µg$280.00
ARCA 100 µg$680.00
ARCA 1 mg$3680.00
Cleancap 20 µg$280.00
CleanCap 100 µg$680.00
CleanCap 1 mg$3680.00
CleanCap 5 X 1 mg$13180.00
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Product Description
Product Name NLS-eCas9-T2A-EGFP mRNA
Description

NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at N terminus. The mutant Cas9 reduces off-target effects by more than 10 folds. EGFP is linked to NLS-eCas9 via T2A peptides

Codon Optimization Human & mouse
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application CRISPR, Genome Enginering
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC
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