NLS-Cre Recombinase Self-Amplifying mRNA, SA-NLS-Cre
Catalog #: EG-313
| Size | Catalog # | Price | Add to Cart |
|---|---|---|---|
| 10 µg | — | $980.00 | |
| 20 µg | — | $1800.00 | |
| 100 µg | — | $9500.00 | |
| View Cart | |||
Product Description
| Product Name | NLS-Cre recombinase self-amplifying mRNA (VEEV), SA-NLS-Cre |
| Description |
The self-amplifying NLS-Cre mRNA (SA-NLS-Cre) encodes four nonstructural proteins (NSP1 to NSP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and human codon-optimized Cre recombinase fused to nuclear localization sequence. Upon entering the cytoplasm, the mRNA is translated to produce replicase complex, which amplifies the original NLS-Cre mRNA template to produce numerous copies of itself (mRNA replication), thus driving robust NLS-Cre protein expression. It can be used for loxP recombination in cell lines and primary cells |
| Codon Optimization | Human & mouse |
| Modifications |
|
| Application | ex vivo LoxP recombination |
| Concentration | ~1 µg/µl |
| Transfection Protocol | Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol |
| QC |
|
| Shipping | Dry ice |
| Storage | -80 oC |
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