Human Klf4-Oct4-Sox2-Glis1-c-Myc (KOSGM) Self-Amplifying mRNA
Catalog #: EG-312
| Size | Catalog # | Price | Add to Cart |
|---|---|---|---|
| 10 µg | — | $298.00 | |
| 20 µg | — | $498.00 | |
| 100 µg | — | $2500.00 | |
| View Cart | |||
Product Description
| Product Name | Human Klf4-Oct4-Sox2-Glis1-c-Myc (KOSGM) Self-Amplifying mRNA |
| Description |
This self-amplifying mRNA encodes four nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and five human transcription factors (OCT4, KLF4, c-MYC, SOX2, GLIS1) linked by four 2A self-cleaving peptides |
| Applications | Efficient, rapid, integration-free generation of pluripotent stem (iPS) cells from human fibroblasts. |
| Modifications |
|
| Concentration | ~1 µg/µl |
| Transfection Protocol | Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol |
| QC | Agarose gel electrophoresis |
| Shipping | Dry ice |
| Storage | -80 oC |
| References | Reprogramming human fibroblasts to pluripotency using modified mRNA. Mandal & Rossi, Nature Protocols 8,568-582 (2013) | doi:10.1038/nprot.nprot.2013.019. |
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