Bst DNA Polymerase, large fragment, 100 U/µl

Catalog #: EG-1084

Size	Catalog #	Price	Add to Cart
4,000 Units	—	$50.00
20,000 Units	—	$200.00
100,000 Units	—	$1000.00
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Product Description

Description	Recombinant Bst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease activity. This enzyme can be used for and rapid amplification of trace amounts of DNA templates for sequencing.
Applications	DNA sequencing through high GC regions Rapid amplification of trace amounts of DNA templates Next Generation sequencing
Source	A recombinant E. coli strain carrying the large fragment of DNA polymerase gene from Bacillus stearothermophilus
Unit Definition	One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C.
Components	Bst DNA Polymerase: 100,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM (NH₄)₂SO₄, 100 mM KCl, 20 mM MgSO₄, and 1.0 % Triton X-100, pH 8.8 @ 25°C
Quality Control	The absence of endonuclease, nicking activity and exonuclease activity is confirmed by appropriate quality tests.
Storage Condition	-20 °C

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