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Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre), Endotoxin-free

Catalog #: EG-1021


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Description

Recombinant Cre recombinase (TAT-Cre) was purified from an HEK293 cell line expressing enhanced form of Cre Recombinase from bacteriophage P1. This Cre recombinase has an N-terminal 6XHis tag, a Tat peptide (GRKKRRQRRRPPAGTSVSL) and an NLS sequence (PKKKRKV). Tat-Cre is the most effective protein in transduction (in vivo) and subsequent recombination compared to other forms of Cre recombinases, e.g., HNC, TCH6, HC, HNCM, CH.

Applications

  • In vitro LoxP recombination for subcloning or vector/clone engineering
  • Transduction into cultured cells including stem cells ex vivo
Source HEK 293 cells
Activity A standard of 100 Units is defined as the amount of Tat-Cre in 1.0 mL of tissue culture medium that is required to induce 90% GFP expression in a HEK 293T loxP reporter cell line assay.
Properties

QC: HEK293T- Cre reporter cell line 80~100% recombination efficiencies.

Figure 1. QC assay of Tat-Cre. Adding  50 and 100 units of Tat-Cre to HEK 293T cells transfected with LoxP-RFP-Stop-LoxP-GFP reporter plasmid DNA.

Endotoxin Levels: < 0.1 EU/ug

Storage -20ºC ~ -80ºC
Shipping dry ice

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