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SARS-CoV-2 Spike Glycoprotein RBD (319-541), United Kingdom Variant, His tag

Catalog #: EG-324


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Cat # EG-324
Description A recombinant form of United Kingdom variant of the spike glycoprotein receptor binding domain (RBD, Arg319-Phe541) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2). This RBD mutant has N501Y mutation in the S glycoprotein RBD as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). It was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography. This recombinant protein contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD with a C-terminal hexa-histidine tag.
Mutations N501Y
Tags
C-terminal hexa-histidine
Purification
FPLC by nickel affinity and ion exchange chromatography
Sequence
1   RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL
51  YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI
101 ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI
151 STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTYGVGYQPY RVVVLSFELL
201 HAPATVCGPK KSTNLVKNKC VNFHHHHHH
Mol Wt
25.96 kDa
Source
HEK 293
Purity
90~95% by SDS-PAGE

Applications

Western blot, ELISA and animal vaccination
Storage Buffer

PBS + 50% Glycerol

Storage -20ºC ~ -80ºC
Shipping dry ice
References


     
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