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Cre Recombinase, HNC

Catalog #: EG-1067


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Description

Recombinant Cre recombinase HNCre was purified from an E. coli strain carrying an engineered plasmid encoding Cre Recombinase from bacteriophage P1 with additional N-terminal 6XHis tag and an NLS sequence (PKKKRKV). This Cre recombinase has lower cell permeability than Tat-Cre, but can be transported into cells using cell permeable peptides and some protein transfection reagents. It is effective in recombination in various cell types after being transported into cells

Applications

  • In vitro LoxP recombination for subcloning or vector/clone engineering
  • Transduction into cultured cells including stem cells ex Vivo
Properties

Molecular Weight: 40 kDa

Endotoxin Levels: < 0.1 EU/ug

Purity: >95% by SDS-PAGE and HPLC analysis

Storage -20ºC ~ 80ºC
Shipping dry ice

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