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DNA Polymerase I (E. coli)
Catalog #:
EG-1054
Please Choose:
Size
1,000 Units ( $50.00 )
5,000 Units ( $118.00 )
Qty:
Description
DNA Polymerase I (
E. coli
) is a mesophilic DNA polymerase that exhibits 5′-3′ DNA synthesis in addition to both 3′→ 5′ and 5′→ 3′ exonuclease activities. The combination of DNA synthesis and 5′→ 3′ nuclease characteristics enable nick-translation during DNA synthesis.
Applications
Nick translation of DNA in conjunction with DNase to obtain probes with a high specific activity
Second strand synthesis of cDNA in conjunction with RNase H
Source
A recombinant
E. coli
strain carrying
E coli
PolA gene.
Unit Definition
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
Components
DNA Polymerase l:
10,000 U/ml in
25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C
10X Reaction Buffer
: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl
2
, 10 mM DTT, pH 7.9 @ 25°C
Quality Control
The absence of endodeoxyribonucleases confirmed by appropriate quality test.
Storage Condition
-20 °C
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