Log In | Shopping Cart | Advanced Search
            
  • Home
  • Career
  • Distributors
  • Contact Us:
  • Email: crm@excellgen.com
Products
Modified mRNA
Encapsulated mRNA
mRNA Enzyme
SARS-CoV-2
Pseudovirus
Popular Products
Cancer Proteins
Antibodies
DNA Binding Proteins
Electrophoresis
Genome Engineering
Polymerases
Recombinases
Transcription Factors
Transfection
Services
Modified mRNA
Custom Cloning
Virus Production
Protein Purification
Protein Expression
Polymerases
 Previous 
 Return to List 
 Next 

Bst DNA Polymerase, large fragment, 8 U/µl

Catalog #: EG-1083


Please Choose:



Qty:

Description

Recombinant Bst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease activity. This enzyme can be used for and rapid amplification of trace amounts of DNA templates for sequencing.

Applications
  • DNA sequencing through high GC regions
  • Rapid amplification of trace amounts of DNA templates
  • Next Generation sequencing
Source A recombinant E. coli strain carrying the large fragment of DNA polymerase gene from Bacillus stearothermophilus
Unit Definition One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C.
Components
  • Bst DNA Polymerase: 8,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C
  • 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100, pH 8.8 @ 25°C
Quality Control The absence of endonuclease, nicking activity and exonuclease activity is confirmed by appropriate quality tests.
Storage Condition

-20 °C


Customers who bought this product also purchased...

ExcellScript M-MLV (MMLV) Reverse Transcriptase RNase H minus
ExcellScript M-MLV (MMLV) Reverse Transcriptase RNase H minus
GeneExpresso™ Max Transfection Reagent
GeneExpresso™ Max Transfection Reagent
Gst DNA Polymerase, large fragment
Gst DNA Polymerase, large fragment
     
 Ask a Question 
 
 Home | Privacy Policy | Terms & Conditions | Online Price Policy | Shipping & Return

Copyright © 2007-2022  Excellgen, Inc. All rights reserved