Human Klf4-Oct4-Sox2-c-Myc (KOSM) mRNA

Catalog #: EG-308

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Product Name	Human Klf4-Oct4-Sox2-c-Myc (KOSM) mRNA
Description	This mRNA will express 4 human transcription factors, OCT4, KLF4, c-MYC, SOX2, LIN28A and RFP. The delivery efficiency can be monitored by fluorescence microscope using these Omega Optical filter sets: QMAX-Yellow, XF108-2, XF101-2, and XF111-2, or TRITC filter
Applications	Reprogramming human fibroblasts to generate induced pluripotent stem (iPS) cells
Modifications	5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1) Pseudo-UTP 5mCTP PolyA: 180 nt
Concentration	~1 μg/μl
Transfection Protocol	Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC	Agarose gel electrophoresis
Shipping	Dry ice
Storage	-80 oC
References	Reprogramming human fibroblasts to pluripotency using modified mRNA. Mandal & Rossi, Nature Protocols 8,568-582 (2013) \| doi:10.1038/nprot.nprot.2013.019.

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