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NLS-FLP Recombinase mRNA, Animal-Free

Catalog #: EG-1044


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Product Name NLS-FLP Recombinase mRNA
Description

This NLS-FLP recombinase mRNA encodes a human codon-optimized Flp recombinase fused to nuclear localization sequence, it can be used for FRT recombination in cell lines and primary cells.

mRNA is made from animal-free components.

Applications

ex vivo FRT recombination
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Fully substituted with Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC
  • Agarose gel electrophoresis
  • Activity assay: HEK293 cells transfected with FRT reporter plasmid
Shipping Dry ice
Storage -80 oC

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