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NLS-eCas9 Self-Amplifying mRNA

Catalog #: EG-322


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Product Name NLS-eCas9 Self-Amplifying mRNA
Description

This self-amplifying mRNA encodes four nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and NLS-eCas9.

NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at both N and C terminus. The mutant Cas9 reduces off-target effects by more than 10 folds.

Modifications
  • 5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application CRISPR, Genome Enginering
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC

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