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NLS-Cre Recombinase Self-Amplifying mRNA, SA-NLS-Cre

Catalog #: EG-313


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Product Name NLS-Cre recombinase self-amplifying mRNA (VEEV), SA-NLS-Cre
Description

The self-amplifying NLS-Cre mRNA (SA-NLS-Cre) encodes four nonstructural proteins (NSP1 to NSP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and human codon-optimized Cre recombinase fused to nuclear localization sequence. Upon entering the cytoplasm, the mRNA is translated to produce replicase complex, which amplifies the original NLS-Cre mRNA template to produce numerous copies of itself (mRNA replication), thus driving robust NLS-Cre protein expression. It can be used for loxP recombination in cell lines and primary cells

Codon Optimization Human & mouse
Modifications
  • 5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application ex vivo LoxP recombination
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC
  • Agarose gel electrophoresis
  • Activity assay: HEK293 cells transfected with LoxP reporter plasmid
Shipping Dry ice
Storage -80 oC

     
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