Log In | Shopping Cart | Advanced Search
            
  • Home
  • Career
  • Distributors
  • Contact Us:
  • Email: crm@excellgen.com
Products
Modified mRNA
Encapsulated mRNA
mRNA Enzyme
SARS-CoV-2
Pseudovirus
Popular Products
Cancer Proteins
Antibodies
DNA Binding Proteins
Electrophoresis
Genome Engineering
Polymerases
Recombinases
Transcription Factors
Transfection
Comsumbles
Services
Modified mRNA
Custom Cloning
Virus Production
Protein Purification
Protein Expression
Modified mRNA
 Previous 
 Return to List 
 Next 

NLS-Cas9-D10A Nickase mRNA

Catalog #: EG-315


Please Choose:



Qty:

Product Name NLS-Cas9-D10A Nickase mRNA
Description

NLS-Cas9-D10A Nickase mRNA encodes a Streptococcus pyogenes SF370 Cas9 protein D10A mutant with nuclear localization signal (NLS) at C terminus. Unlike the wild-type CAS9, which cleaves the double strand DNA generating two break sites based on its two active domains, the NLS-Cas9-D10A Nickase has only one active domain, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand break. This mutant mRNA favors homology-directed repair and decreases the occurrence of non-homologous end joining.

Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application CRISPR, Genome Enginering
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC

     
 Ask a Question 
 
 Home | Privacy Policy | Terms & Conditions | Online Price Policy | Shipping & Return

Copyright © 2007-2023  Excellgen, Inc. All rights reserved