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Cas9 recombinant protein, NLS-Cas9 , S. pyogenes
Catalog #:
EG-209
$395.00
Qty:
Size
100 µg
Description
Recombinant Cas9 protein (∼160 KD) can be used for genome engineering experiments. When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection. This protein is purified from E. coli strain expressing codon optimized Cas9, it contains N-terminal TAT (GRKKRRQRRRPPAGTSV) and an Simian virus 40 (SV40) nuclear localization sequence (NLS, KKKRKV) , 1 C-terminal NLS, and 1 C-terminal 6-His tag
Applications
In vitro DNA digestion
Genome editing
Properties
Purity
: > 95% as determined by SDS-PAGE and Coomassie blue staining
MW
: ~160 KDa
Storage Buffer
: 10 mM HEPES, pH7.4, 300 mM NaCl,0.1 mM EDTA,1 mM DTT, 50% glycerol.
Stability
2 years at -80
o
C. 1 years at -20
o
C. 1 month at 4
o
C
Storage
4 ~ -20ºC
Shipping
dry ice
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