Cas9 recombinant protein, NLS-Cas9 , S. pyogenes

Catalog #: EG-209

Qty:

Size	100 µg
Description	Recombinant Cas9 protein (∼160 KD) can be used for genome engineering experiments. When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection. This protein is purified from E. coli strain expressing codon optimized Cas9, it contains N-terminal TAT (GRKKRRQRRRPPAGTSV) and an Simian virus 40 (SV40) nuclear localization sequence (NLS, KKKRKV) , 1 C-terminal NLS, and 1 C-terminal 6-His tag
Applications	In vitro DNA digestion Genome editing
Properties	Purity: > 95% as determined by SDS-PAGE and Coomassie blue staining MW: ~160 KDa Storage Buffer: 10 mM HEPES, pH7.4, 300 mM NaCl,0.1 mM EDTA,1 mM DTT, 50% glycerol.
Stability	2 years at -80 ^oC. 1 years at -20 ^oC. 1 month at 4 ^oC
Storage	4 ~ -20ºC
Shipping	dry ice

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