Log In
|
Shopping Cart
|
Advanced Search
Home
Career
Distributors
Contact Us:
Phone: 855-278-2398
Email: crm@excellgen.com
Products
New Products
Antibodies
Cancer Proteins
Cytokines
DNA Binding Proteins
DNA Modifying Enzymes
Electrophoresis
Genome Engineering
Glucosyltransferases
Glycosylases
Kinases and Phosphatases
Ligases
Lysozymes
Nucleases
Nuclear Receptors
PCR & qPCR
Peptides
Polymerases
Proteases
Recombinant Proteins
Recombinases
Restriction Enzymes
RNA Enzymes
Stem Cell Reagents
5' Capped mRNA
Transcription Factors
Transfection
Topoisomerases
Services
Conjugation
Custom Cloning
Protein Expression
Genome Engineering
Previous
Return to List
Next
Cas9 recombinant protein, NLS-Cas9 , S. pyogenes
Catalog #:
EG-209
$198.00
Qty:
Size
100 µg
Description
Recombinant Cas9 protein (∼160 KD) can be used for genome engineering experiments. When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection. This protein is purified from E. coli strain expressing codon optimized Cas9, it contains N-terminal TAT (GRKKRRQRRRPPAGTSV) and an Simian virus 40 (SV40) nuclear localization sequence (NLS, KKKRKV) , 1 C-terminal NLS, and 1 C-terminal 6-His tag
Applications
In vitro DNA digestion
Genome editing
Properties
Purity
: > 95% as determined by SDS-PAGE and Coomassie blue staining
MW
: ~160 KDa
Storage Buffer
: 10 mM HEPES, pH7.4, 300 mM NaCl,0.1 mM EDTA,1 mM DTT, 50% glycerol.
Stability
2 years at -80
o
C. 1 years at -20
o
C. 1 month at 4
o
C
Storage
4 ~ -20ºC
Shipping
dry ice
Ask a Question
Home
|
Privacy Policy
|
Terms & Conditions
|
Online Price Policy
|
Shipping & Return
Copyright © 2007-2019 Excellgen, Inc. All rights reserved
