| Product Name
|
Ribonuclease-Resistant SARS-CoV-2 mRNA qPCR Control
|
Description
|
Ribonuclease-Resistant SARS-CoV-2 mRNA is an
mRNA-protein complex,
produced in
Escherichia coli by the induction of an expression plasmid that encodes
both the MS2 coat protein and SARS-CoV-2 mRNA sequence. Upon
IPTG induction, both SARS-CoV-2 mRNA and MS2 coat protein are synthesized and
assembled
into pseudoviral nanoparticles. The
purified MS2 coat protein-SARS-CoV-2 mRNA complex is DNA-free and protected from degradation
by RNase. The MS2 SARS-CoV-2 mRNA nanoparticles are stable at 4 °C or room temperature for up to 6 months
|
| Sequence
|
AUAAUGGACC CCAAAAUCAG CGAAAUGCAC CCCGCAUUAC GUUUGGUGGA CCCUCAGAUU 60
CAACUGGCAG UAACCAGAAU GGAGAAGAAC UGAUUACAAA CAUUGGCCGC AAAUUGCACA 120
AUUUGCCCCC AGCGCUUCAG CGUUCUUCGG AAUGUCGCGC AUUGGCGUUG CAACUGAGGG 180
AGCCUUGAAU ACACCAAAAG AUCACAUUGG CACCCGCAAU CCUGCUAACA AUGCUGCAAU 240
CGUGCUACAA CUUCCUCAGU GUUUGCAGAU UUGGACCUGC GAGCGGGUUC UGACCUGAAG 300
GCUCUGCGCG GACUUGUGGA GACAGCCGCU CACCUUGGCU AUUGAGUGAA AUGGUCAUGU 360
GUGGCGGUUC ACUAUAUGUU AAACCAGGUG GAACCUCAUC AGGAGAUGCC ACAACUGCUU 420
AUGCUAAUAG UGUUUUUAAC AUUUGUCAAG CUGAAGAGAC AGGUACGUUA AUAGUUAAUA 480
GCGUACUUCU UUUUCUUGCU UUCGUGGUAU UCUUGCUAGU UACACUAGCC AUCCUUACUG 540
CGCUUCGAUU GUGUGCGUAC UGCUGCAAUA UUGUUAACGU GAG 583
|
Titer
|
10^10/μL copies of mRNA
|
| Size
|
100 μl, 1X10^12 copies of mRNA
|
Format
|
Liquid
|
QC
|
qPCR, Agarose Gel
|
| Applications |
Quantitative RNA standard for qPCR
|
Storage
|
-80 °C
|
Stability
|
Liquid at -80 °C: 2 years; 4°C ~ 40°C: 6 months
|
