Catalog #: EG-198
The Tth MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This 91.8-kDa protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Tth MutS is active at temperature between 0 to 75°C. Since Tth MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels or on a solid phase such as Ni agarose or magnetic Ni-NTA particles.
~ 95% as determined by SDS-PAGE
Thermus thermophilus MutS DNA mismatch repair protein
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4. One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36.
5. Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods - 4, 257 - 262 (2007) doi:10.1038/nmeth1007
