Catalog #: EG-156
Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex DNA. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease exhibits low activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease has no activity at nicks and limited activity at gaps in DNA
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C
The absence of endodeoxyribonucleases is confirmed by appropriate quality test. Functionally tested for generating single-stranded DNA from double-stranded PCR product.
-20 °C