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Single-Stranded DNA Binding Protein (E. coli SSB)

Catalog #: EG-156


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Description

Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex DNA. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease exhibits low activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease has no activity at nicks and limited activity at gaps in DNA

Applications
  • Generating single-stranded PCR products for use in:
  • DNA sequencing
  • Analysis of DNA single-strand conformation polymorphism (SSCP)
  • Rolling circle amplification
  • Producing single-stranded DNA from double-stranded DNA fragments
  • Cloning of PCR products
Source An E. coli strain that carries the Phage lambda exo gene
Unit Definition

One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C

Components
  • Lambda Exonuclease: 5,000 U/ml in 25 mM Tris-HCl, 50 mM NaCl, 1.0 mM DTT, 0.1% mM EDTA, 50% Glycerol, pH 7.5 @ 25°C
  • 10X Reaction Buffer: 670 mM Glycine-KOH, 25 mM MgCl2, pH 9.4 @ 25°C
Quality Control

The absence of endodeoxyribonucleases is confirmed by appropriate quality test.
Functionally tested for generating single-stranded DNA from double-stranded PCR product.

Storage Condition

-20 °C


     
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