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Custom Cloning

Catalog #: EG-34


$3,500.00

Qty:

Cloning and Gene Synthesis:

1) Cloning of PCR products by restriction enzyme digestion and ligation, TOPO (TA) cloning, In-fusion cloning, Gateway Cloning and Ligation Independent Cloning (LIC).

2) Cloning of your genes into baculovirus, lentiviral, adenoviral and adeno-associated virus vectors. Custom production of high titer integrative or non-integrative lentivirus (108~109 TU/ml), adenovirus (>1012 PFU/ml) and adeno-associated virus (>1013 GC/ml).

3) Gene synthesis (100 bp to 5 kb) and clone synthetic gene into a vector of your choice.

5) Construction of fusion proteins:

* Common Expression and Purification Tags: Poly-histidine Tag (6XHis, 8XHis and 9XHis), Maltose binding protein (MBP), Glutathione-S-transferase (GST), Chitin binding protein (CBP), Thioredoxin, NusA.

 

* Tandem Affinity Purification Tags: 6XHis.GST, 6XHis.MBP.

 

* Monomeric Fluorescent Proteins: Fusion of your gene to the N- or C-terminus of EGFP, BFP, CPF, YFP, Red Fluorescent Proteins (RFP), infrared fluorescent protein (IFP), Infrared red fluorescent protein (IRFP), large Stokes shifts RFPs.

 

* Luciferase and secreted luciferase alone or fused to monomeric fluorescent proteins using self-cleaving 2A peptides (F2A, T2A, E2A, and P2A).

 

* Detection Tags: c-myc, HA, Flag, 3XFlag, Halo, Strep, T7, S-Tag, Streptavidin-Binding Peptide (SBP), V5 Epitope, Xpress Epitope.

 

* Color Tags: Soluble Red Tag, cytochrome b5, 13 kDa; Yellow Tag: flavin mononucleotide (FMN) domain of human cytochrome P450 reductase, 21-kDa.

 

* Secretion Signal Peptides: for protein expression in yeast, insect cells, and 293/CHO cells.

 

5) Site-directed mutagenesis and multi site-directed mutagenesis.

6) Preparation of endotoxin-free plasmid DNA.

 


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