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SV40 T-Antigen (Simian Virus 40 Large Antigen)
Catalog #:
RP-116
$260.00
Qty:
Product Name
SV40 T-Antigen (Simian Virus 40 Large Antigen)
Size
5,000 U
Description
The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth (1-4). A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain (5,6). The p53-interacting domain is also essential for binding p300/CBP (7). Phosphorylation of TAg by several kinases may regulate its function both positively and negatively (8).
Applications
DNA unwinding assay
DNA replication assay
DNA binding assays
protein-protein interaction assays
cell growth assay
Source
Baculovirus-insect cell
Fusion Tag
6X His
Purification Method
FPLC
Unit Definition (Activity)
1 unit equals to 1 nanogram of purified protein. 50-500 units are sufficient for an in vitro unwinding assay and 100 units are sufficient for a protein-protein interaction assay.
Purity
~ 95% as determined by SDS-PAGE
Gene ID
11039991
Accession #
AAG39214.1
Gene Name
SV-12_gp5 large T antigen
Gene Symbol
SV-12_gp5
MW
83.3 kDa
Protein Sequence
MDKVLNREES LQLMDLLGLE RSAWGNIPLM RKAYLKKCKE FHPDKGGDEE KMKKMNTLYK
KMEDGVKYAH QPDFGGFWDA TEIPTYGTDE WEQWWNAFNE ENLFCSEEMP SSDDEATADS
QHSTPPKKKR KVEDPKDFPS ELLSFLSHAV FSNRTLACFA IYTTKEKAAL LYKKIMEKYS
VTFISRHNSY NHNILFFLTP HRHRVSAINN YAQKLCTFSF LICKGVNKEY LMYSALTRDP
FSVIEESLPG GLKEHDFNPE EAEETKQVSW KLVTEYAMET KCDDVLLLLG MYLEFQYSFE
MCLKCIKKEQ PSHYKYHEKH YANAAIFADS KNQKTICQQA VDTVLAKKRV DSLQLTREQM
LTNRFNDLLD RMDIMFGSTG SADIEEWMAG VAWLHCLLPK MDSVVYDFLK CMVYNIPKKR
YWLFKGPIDS GKTTLAAALL ELCGGKALNV NLPLDRLNFE LGVAIDQFLV VFEDVKGTGG
ESRDLPSGQG INNLDNLRDY LDGSVKVNLE KKHLNKRTQI FPPGIVTMNE YSVPKTLQAR
FVKQIDFRPK DYLKHCLERS EFLLEKRIIQ SGIALLLMLI WYRPVAEFAQ SIQSRIVEWK
ERLDKEFSLS VYQKMKFNVA MGIGVLDWLR NSDDDDEDSQ ENADKNEDGG EKNMEDSGHE
TGIDSQSQGS FQAPQSSQSV HDHNQPYHIC RGFTCFKKPP TPPPEPET
Storage Buffer
20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol
Storage
-20 to -80 °C.
Shipping
4°C or dry ice
References
1. Fanning E., (1992) J. Viol. 66, 1289-1293.
2. Pan ZQ. et al., (1996) J. Biol. Chem. 271, 22111-22116.
3. Chao, HA. et al., (2000) Mol. Cell. Biol. 20, 7624-7633.
4. Quartin, RS. et al., (1994) J. Viol. 68, 1334-1341.
5. Marton, A. et al., (1993) Nucleic Acids Res. 21, 1689-1695.
6. Kim, HY. et al., (2001) EMBO J. 20, 295-304.
7. Lill, NL. et al., (1997) Nature 387, 823-827.
8. Moarefi, IF. et al., (1993) J. Viol. 67, 4992-5002.
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