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Cancer Proteins

SV40 T-Antigen (Simian Virus 40 Large Antigen)

Catalog #: RP-116

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Product Name	SV40 T-Antigen (Simian Virus 40 Large Antigen)
Size	5,000 U
Description	The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth (1-4). A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain (5,6). The p53-interacting domain is also essential for binding p300/CBP (7). Phosphorylation of TAg by several kinases may regulate its function both positively and negatively (8).
Applications	DNA unwinding assay DNA replication assay DNA binding assays protein-protein interaction assays cell growth assay
Source	Baculovirus-insect cell
Fusion Tag	6X His
Purification Method	FPLC
Unit Definition (Activity)	1 unit equals to 1 nanogram of purified protein. 50-500 units are sufficient for an in vitro unwinding assay and 100 units are sufficient for a protein-protein interaction assay.
Purity	~ 95% as determined by SDS-PAGE
Gene ID	11039991
Accession #	AAG39214.1
Gene Name	SV-12_gp5 large T antigen
Gene Symbol	SV-12_gp5
MW	83.3 kDa
Protein Sequence	MDKVLNREES LQLMDLLGLE RSAWGNIPLM RKAYLKKCKE FHPDKGGDEE KMKKMNTLYK KMEDGVKYAH QPDFGGFWDA TEIPTYGTDE WEQWWNAFNE ENLFCSEEMP SSDDEATADS QHSTPPKKKR KVEDPKDFPS ELLSFLSHAV FSNRTLACFA IYTTKEKAAL LYKKIMEKYS VTFISRHNSY NHNILFFLTP HRHRVSAINN YAQKLCTFSF LICKGVNKEY LMYSALTRDP FSVIEESLPG GLKEHDFNPE EAEETKQVSW KLVTEYAMET KCDDVLLLLG MYLEFQYSFE MCLKCIKKEQ PSHYKYHEKH YANAAIFADS KNQKTICQQA VDTVLAKKRV DSLQLTREQM LTNRFNDLLD RMDIMFGSTG SADIEEWMAG VAWLHCLLPK MDSVVYDFLK CMVYNIPKKR YWLFKGPIDS GKTTLAAALL ELCGGKALNV NLPLDRLNFE LGVAIDQFLV VFEDVKGTGG ESRDLPSGQG INNLDNLRDY LDGSVKVNLE KKHLNKRTQI FPPGIVTMNE YSVPKTLQAR FVKQIDFRPK DYLKHCLERS EFLLEKRIIQ SGIALLLMLI WYRPVAEFAQ SIQSRIVEWK ERLDKEFSLS VYQKMKFNVA MGIGVLDWLR NSDDDDEDSQ ENADKNEDGG EKNMEDSGHE TGIDSQSQGS FQAPQSSQSV HDHNQPYHIC RGFTCFKKPP TPPPEPET
Storage Buffer	20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol
Storage	-20 to -80 °C.
Shipping	4°C or dry ice
References	1. Fanning E., (1992) J. Viol. 66, 1289-1293. 2. Pan ZQ. et al., (1996) J. Biol. Chem. 271, 22111-22116. 3. Chao, HA. et al., (2000) Mol. Cell. Biol. 20, 7624-7633. 4. Quartin, RS. et al., (1994) J. Viol. 68, 1334-1341. 5. Marton, A. et al., (1993) Nucleic Acids Res. 21, 1689-1695. 6. Kim, HY. et al., (2001) EMBO J. 20, 295-304. 7. Lill, NL. et al., (1997) Nature 387, 823-827. 8. Moarefi, IF. et al., (1993) J. Viol. 67, 4992-5002.

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