LvExpress^TM Lentivirus Production and Purification

Advantages:

• Lightning Fast Cloning: Transfer GOI from Gateway donor vector to lentivirus vector in just 1 hour using Gateway LR Clonase. The latest hORFeome (v7.1) collection has 18,414 human ORFs representing at least 15,415 human genes. We are currently subcloning this set of libary into adenovirus and lentivirus vectors.
• Reliable:  Generate high titer lentivirus in only 5 days.
• Scalable:  Lentivirus production can be scaled up to 1 or 10 L of cultured cells.
• Fast Turnaround: Maximum 3 weeks are required for cloning,  generation of high titer lentivirus and virus purification.
• Strong eCMV Promoter: Exceptional high level protein expression using eCMV promoter in most cell types including dividing and non-dividing or primary cells.
• High Titer: from 10⁶ to 10¹⁰ TU/ml
• High Yield: up to 10¹¹ TU
• High Purity: Purify virus by FPLC or ultracentrifugation.
• Flexiable: shRNA for gene knockdown; cDNA: high level protein expression using eCMV promoter.
• Promoter of Your Choice: hCMV, EF-1alpha, eCMV, CAG, etc.
• Reporters: EGFP, monomeric GFP, RFP, iREP, Luciferases.

LvExpress^TM Lentivirus Services:

1, Clone gene of interest into a lentivirus vector.
2, Large scale lentivirus production in 293 cells.
3. Lentivirus purification by ultracentrifugation and FPLC.
4, Titer determination.

Required Materials:  Sequence of gene of interest or shRNA, or plasmid DNA containing gene of interest.

Turnaround Time:

• Full Service: ~ 2 to 3 weeks.
• Customized Projects: ~ 1  to 3 weeks.

Deliverable: Purified high titer (up to 1X10¹⁰ TU/ml) lentivirus.

Please download viral service request form