AdExpress^TM Adenovirus Production, Amplification and Purification

AdExpress^TM Adenovirus System offers the following advantages

• Lightning Fast Cloning: Transfer GOI from Gateway donor vector to adenovirus shuttle vector in just 1 hour using Gateway LR Clonase. The latest hORFeome (v7.1) collection has 18,414 human ORFs representing at least 15,415 human genes. This set of libary or indvidual clone can be purchased from Dana-Farber/Harvard Cancer Center  or ASU's DNASU Plasmid Repository. Other human or mouse Gateway clones are available from Invitrogen (Ultimate™ ORF)  or GeneCopoeia.
• Reliable:  Generate high titer crude adenovirus stock in only 5 days.
• Scalable:  Adenovirus production can be scaled up to 1 or 10 L of cultured cells.
• Flexiable: shRNA for gene knockdown; high level protein expression using CMV5 promoter.
• Fast Turnaround: Maximum 2 weeks are required for cloning and generation of high titer crude adenovirus stock. Another 2 weeks are needed for large scale amplification and purification.
• RCA-Free: Special cell lines can be used to produce high quality replication-competent-free adenovirus.
  
• High Titer: Up to 10¹³ particles/ml or 10¹² PFU/ml.
• High Purity: Adenovirus purification by CsCl ultracentrifugation and FPLC.
  
• Promoter of Your Choice: mCMV, hCMV, EF-1alpha, CAG, etc.
• Strong eCMV Promoter: Exceptional high level protein expression using eCMV promoter in most cell types including dividing and non-dividing or primary cells.
• Reporters: EGFP, monomeric GFP, RFP, iRFP, Luciferases.

AdExpress^TM Adenovirus Services:

1, Clone gene of interest directly into an adenovirus vector.
2, Produce crude adenovirus stock.
3, Large scale amplification, adenovirus purification by CsCl banding (ultracentrifugation) and FPLC.
4, Titer determination.
5. RCA assay.
6, Protein production in suspension cultured cells.

Required Materials:  Sequence of gene of interest or shRNA, or plasmid DNA containing gene of interest.

Turnaround Time:

• Full Service: ~4 to 7 weeks.
• Customized Projects: ~ 1  to 7 weeks.

Deliverable: Purified high titer (up to 1X10¹³ viral particles/ml, or 1X10¹² PFU/ml) adenovirus.

 Please download viral service request form