Ad2Protein™ Protein Production in CHO or Hela Cells from Adenovirus
- Transient Expression of functional proteins in suspension cultured CHO or Hela cells
- Strong eCMV promoter to produce and purify up to 100 mg protein in 4~6 weeks
- Laboratory scale from 100 ml to 3 L of cultured cells.
Pilot Expression
A pilot project will be carried out before starting a large-scale protein production service. Optimal parameters for protein expression will be determined for each individual protein.
- Infection of HeLa cells using previously generated recombinant adenovirus with three different MOI (multiplicity of infection).
- Harvesting infected cells at two time points, typically at 48 and 72 hours post-infection.
Time Required: 2-3 weeks.
Large-Scale Protein Production
Protein expression in CHO or HeLa cells, according to characteristics of the recombinant adenovirus or customer's specifications.
Deliverables
- 1 to 100 mg purified (> 95% purity) His-Fc fusion protein, cleavable by TEV protease or enterokinase.
- QC Data
Required Materials
- Plasmid containing gene of interest plus complete vector sequence in electronic format
- or protein sequence for gene synthesis (with additional price for gene synthesis)
- or accession number
Quotation and Ordering
To request a quotation, please request a quotation form from customer services crm@excellgen.com
To order, please send the completed quotation form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone.
If submitting samples, please mail them together with a hard copy of the completed quotation form to: Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743.
240-454-9768
