Custom Protein Purification Services using Akta FPLC Systems

Excellgen's scientists have average of 15 years experience in molecular biology, protein expression and purification.  Our team is proficient in molecular cloning, producing and purifying of recombinant proteins in a variety of expression systems including cell-free protein synthesis,  E. Coli, yeasts, baculovirus/insect cells and mammalian cells (CHO and 293). We have ÄKTAExplore and ÄKTAPurifier FPLC systems, ~ 30 large GE Pharmacia prepacked  columns (gel filtration, hydrophobic, ion exchange,  desalting) and numerous affinity columns. By combining state-of-the-art fementation and ÄKTA FPLC chromatography systems, we can produce quantities of pure recombinant proteins ranging from miligrams to a few grams. 

Excellgen offers custom protein purification services:

• Protein purification using ÄKTAPurifier FPLC systems with columns including affinity,  ion-exchange , hydrophobic interaction and size exclusion
• Affinity chromatography purification of proteins fused with one of the following tags or proteins
  
  • 6X histidine
  • GST
  • MBP
  • Strep(II)
  • Protein A
  • biotin
  • E-tag
  • calmodulin-binding peptide
• Monoclonal and polyclonal antibody purification using  Pharmacia protein A/G columns
• Removal of tags or fusion protein by protease digestion (if protease site is available) followed by FPLC purification
• QC
  • Purity analysis by SDS-PAGE and Coomassie staining
  • Western blot analysis if antibody is available
  • Protein concentration determination

• Purified  protein, > 95% purity
• QC Data
• Chromatograms

Examples of Supported Sample Media

• Tissue culture supernatant
• Cell extract or homogenates
• Tissue extracts or homogenates
• Biological fluids (serum, plasma, urine, CSF, saliva, BALF)

Required Materials

• Sample(s)
• Protein information (sequence, purification tag, buffer, salt concentration, protein expression level or concentration, solubility etc)
• Antibodies if common purification tag is not used

Quotation and Ordering


To request a quotation, please request a quotation form from customer services crm@excellgen.com

To order, please send the completed quotation form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone.

If submitting samples, please mail them together with a hard copy of the completed quotation form to: Protein Services, Excellgen, 847 Quince Orchard Blvd, Suite H,  Gaithersburg, MD, 20878, US.