Excellgen's scientists have average of 15 years experience in molecular biology, protein expression and purification. Our team is proficient in molecular cloning, producing and purifying of recombinant proteins in a variety of expression systems including cell-free protein synthesis, E. Coli, yeasts, baculovirus/insect cells and mammalian cells (CHO and 293). We have ÄKTAExplore and ÄKTAPurifier FPLC systems, ~ 30 large GE Pharmacia prepacked columns (gel filtration, hydrophobic, ion exchange, desalting) and numerous affinity columns. By combining state-of-the-art fementation and ÄKTA FPLC chromatography systems, we can produce quantities of pure recombinant proteins ranging from miligrams to a few grams.
Excellgen offers custom protein purification services:
- Protein purification using ÄKTAPurifier FPLC systems with columns including affinity, ion-exchange , hydrophobic interaction and size exclusion
- Affinity chromatography purification of proteins fused with one of the following tags or proteins
- 6X histidine
- GST
- MBP
- Strep(II)
- Protein A
- biotin
- E-tag
- calmodulin-binding peptide
- Monoclonal and polyclonal antibody purification using Pharmacia protein A/G columns
- Removal of tags or fusion protein by protease digestion (if protease site is available) followed by FPLC purification
- QC
- Purity analysis by SDS-PAGE and Coomassie staining
- Western blot analysis if antibody is available
- Protein concentration determination
Deliverables
- Purified protein, > 95% purity
- QC Data
- Chromatograms
Examples of Supported Sample Media
- Tissue culture supernatant
- Cell extract or homogenates
- Tissue extracts or homogenates
- Biological fluids (serum, plasma, urine, CSF, saliva, BALF)
Required Materials
- Sample(s)
- Protein information (sequence, purification tag, buffer, salt concentration, protein expression level or concentration, solubility etc)
- Antibodies if common purification tag is not used
Quotation and Ordering
To request a quotation, please request a quotation form from customer services crm@excellgen.com
To order, please send the completed quotation form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone.
If submitting samples, please mail them together with a hard copy of the completed quotation form to: Protein Services, Excellgen, 847 Quince Orchard Blvd, Suite H, Gaithersburg, MD, 20878, US.
800-338-6318
