Excellgen offers custom protein purification services:
- Protein purification using ÄKTAPurifier FPLC systems with columns including affinity, ion-exchange , hydrophobic interaction and size exclusion
- Affinity chromatography purification of proteins fused with one of the following tags or proteins
- Fc
- 6X histidine
- GST
- MBP
- Strep(II)
- Protein A
- Biotin
- E-tag
- Calmodulin-binding peptide
- Monoclonal and polyclonal antibody purification using Pharmacia protein A/G columns
- Removal of tags or fusion protein by protease digestion (if protease site is available) followed by FPLC purification
- QC
- Purity analysis by SDS-PAGE and Coomassie staining
- Western blot analysis if antibody is available
- Protein concentration determination
Deliverables
- Purified protein, > 95% purity
- QC Data
- Chromatograms
Examples of Supported Sample Media
- Tissue culture supernatant
- Cell pellet
- Cell extract or homogenates
- Tissue extracts or homogenates
- Biological fluids (serum, plasma, urine, CSF, saliva, BALF)
Required Materials
- Sample(s)
- Protein information (sequence, purification tag, buffer, salt concentration, protein expression level or concentration, PI, solubility etc)
- Antibodies if common purification tag is not used
Quotation and Ordering
To request a quotation, please request a quotation form from customer services crm@excellgen.com
To order, please send the completed quotation form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone.
If submitting samples, please mail them together with a hard copy of the completed quotation form to: Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743.
240-454-9768
