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Schizosaccharomyces pombe Poly(U) Polymerase

Catalog #: EG-151


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Description

S. pombe Poly(U) (Poly(A)) Polymerase uses ATP as a substrate for template-independent addition of UMP from UTP or AMP from ATP to the 3´ hydroxyl termini of RNA molecules.

Applications
  • Labeling of RNA with UTP
  • Poly(U) and Poly(A) tailing of RNA for cloning
  • Enhance mRNA stability and increase translation efficiency of in vitro synthesized mRNA transferred into eukaryotic cells
  • Poly (A) tailing of 2' O-Me modified 3' ends
Source A recombinant E. coli strain carrying yeast (Schizosaccharomyces pombe) Poly(A) Polymerase gene Cid1
Unit Definition 1 unit is defined as the amount of enzyme that will incorporate 1 nmol of UMP into into RNA in 50 µl in 10 minutes at 37°C.
Components
  • S. pombe Poly(U) Polymerase: 20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.5 @ 25°C
  • 10X Reaction Buffer: 100 mM Tris-HCl,500 mM NaCl, 100 mM MgCl2,10 mM DTT, pH 7.9 @ 25°C
Quality Control The absence of endonuclease, nicking activity, exonuclease and RNAse activity is confirmed by appropriate quality tests.
Storage Condition

-20 °C

 


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