Schizosaccharomyces pombe Poly(U) Polymerase

S. pombe Poly(U) (Poly(A)) Polymerase uses ATP as a substrate for template-independent addition of UMP from UTP or AMP from ATP to the 3´ hydroxyl termini of RNA molecules.

• Labeling of RNA with UTP
• Poly(U) and Poly(A) tailing of RNA for cloning
• Enhance mRNA stability and increase translation efficiency of in vitro synthesized mRNA transferred into eukaryotic cells
• Poly (A) tailing of 2' O-Me modified 3' ends

• S. pombe Poly(U) Polymerase: 2,000 U/ml in 20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.5 @ 25°C
• 10X Reaction Buffer: 100 mM Tris-HCl,500 mM NaCl, 100 mM MgCl2,10 mM DTT, pH 7.9 @ 25°C

-20 °C