Catalog #: EG-1056
Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease (nick-translation) activity, and proofreading (3′→ 5′) exonuclease activity due to two amino acids mutations (D355A, E357A).
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
-20 °C