Log In | Shopping Cart | Advanced Search
            
  • Home
  • Career
  • Distributors
  • Contact Us:
  • Phone: 855-278-2398
  • Email: crm@excellgen.com
Products
New Products
Antibodies
Cancer Proteins
Cytokines
DNA Binding Proteins
DNA Modifying Enzymes
Electrophoresis
Genome Engineering
Glucosyltransferases
Glycosylases
Kinases and Phosphatases
Ligases
Lysozymes
Nucleases
Nuclear Receptors
PCR & qPCR
Peptides
Polymerases
Proteases
Recombinant Proteins
Recombinases
Restriction Enzymes
RNA Enzymes
Stem Cell Reagents
5' Capped mRNA
Transcription Factors
Transfection
Topoisomerases
Services
Conjugation
Custom Cloning
Protein Expression
Polymerases
 Previous 
 Return to List 
 Next 

Bst DNA Polymerase, large fragment, 8 U/µl

Catalog #: EG-1083


Please Choose:



Qty:

Description

Recombinant Bst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease activity. This enzyme can be used for and rapid amplification of trace amounts of DNA templates for sequencing.

Applications
  • DNA sequencing through high GC regions
  • Rapid amplification of trace amounts of DNA templates
  • Next Generation sequencing
Source A recombinant E. coli strain carrying the large fragment of DNA polymerase gene from Bacillus stearothermophilus
Unit Definition One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C.
Components
  • Bst DNA Polymerase: 8,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C
  • 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100, pH 8.8 @ 25°C
Quality Control The absence of endonuclease, nicking activity and exonuclease activity is confirmed by appropriate quality tests.
Storage Condition

-20 °C


Customers who bought this product also purchased...

Gst DNA Polymerase, large fragment
Gst DNA Polymerase, large fragment
     
 Ask a Question 
 
 Home | Privacy Policy | Terms & Conditions | Online Price Policy | Shipping & Return

Copyright © 2007-2018  Excellgen, Inc. All rights reserved