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3C HiFi DNA Polymerase
Catalog #:
EG-1027
Please Choose:
Size
250 Units ( $188.00 )
1,000 Units ( $680.00 )
Qty:
Description
Recombinant High Fidelity 3C Thermostable DNA Polymerase (3C HiFi) is purified from
E. coli
transformed with an engineered DNA polymerase gene similar to a thermostable DNA polymerase from
Thermococcus
. 3C HiFi can amplify target DNA up to 20 kb. The processivity and elongation rate of 3C HiFi are at least 5 times higher than that of Pfu DNA polymerase.
Application
High fidelity PCR amplification
Blunt-end PCR cloning
Source
A recombinant
E. coli
strain carrying an engineered thermostable DNA polymerase
Unit Definition
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material for 30 min at 72°C.
Components
3C HiFi Polymerase
: 5 units in storage buffer, 20 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM Sodium Phosphate, 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% (v/v) glycerol
10X Reaction Buffer
.
Storage Condition
-20 °C
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